V_appUB -3.2[V] Applied voltage, upper buffer inlet V_appS -1[V] Applied voltage, sample inlet u_a 0.2[mm/s] Average velocity, sample inlet w_a 0.67[mm/s] Average velocity, buffer inlets D 1e-9[m^2/s] Sample ion diffusivity T 298[K] Temperature c_in 0.05*3.5[g/l]/(22+35)[g/mol] Sample ion concentration, inlet z_c -1 Charge number