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Isoelectric Separation

Application ID: 10626

This example applies the Transport of Diluted Species, Electrostatics, and Laminar Flow interfaces to model isoelectric separation in a free flow electrophoresis device. A stream containing six different ionic species is shown to be divided into pure component streams by means of migrative transport in an electric field.

Free flow electrophoresis can separate macromolecules such as proteins, based on their mobility perpendicular to the flow of the carrier fluid. If, in addition, a pH gradient is applied across the carrier flow, then molecules can be focussed along their isoelectric points. The isoelectric point is the pH at which the molecule has zero net charge. Molecules with a positive net charge will travel in the direction of the electric field, along the pH gradient, until they reach the isoelectric point. At this instance the migrative transport is switched off as the molecules net charge is zero. Similarly, anionic species travel in the direction opposite of the electric field.

This application was built using the following:

Chemical Reaction Engineering Module

The combination of COMSOL® products required to model your application depends on the physics interfaces that define it. Particular physics interfaces may be common to several products (see the Specification Chart for more details). To determine the right combination of products for your project, you should evaluate all of your needs in light of each product's capabilities, consultation with the COMSOL Sales and Support teams, and the use of an evaluation license.